Summary
Type: SPE
Application #: 302250
Catalog#: 730003 - SPE Cartridges, Chromabond, C18, 3ml, PP, 500mg, 50/pk
Detection: Analytes were converted to their PFB ester TMS ether derivatives using PFB bromide and BSTFA (100µl) by standard derivatisation procedures. Reversed-phase high-performance liquid chromatography (HPLC)
Conditions: Column conditioning: with 10 ml of methanol and 3 ml of 0,05 M HCOOH
Sample application: The prepared samples were applied to ODS cartridges.
Column washing: with 20 ml of water and 2,5 ml of heptane
Elution: with 2 ml of ethyl acetate
Authors: Schwedhelm E Tsikas D Durand Th Gutzki F-M Guy A Rossi J-C Fröhlich J C
Source: Journal of Chromatography B 744 (2000) 99-112